C. elegans live/dead assay from an anti-infective screen (BBBC010v1, Broad Bioimage Benchmark Collection). 100 wells of a 384-well control plate, each imaged in brightfield and GFP (200 images, 696x520, 16-bit TIFF), with 1,407 hand-corrected per-worm masks converted to polygon annotations for overlapping-worm instance segmentation; each worm polygon is placed on both channel images of its well, giving 2,814 polygons. Columns 1-12 are ampicillin-treated positive controls (mostly live worms) and columns 13-24 untreated negative controls (mostly dead), carried as two classes. License: CC0 1.0 (public domain). Source: https://bbbc.broadinstitute.org/BBBC010. Please cite: C. elegans infection live/dead image set version 1 provided by Fred Ausubel, available from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012]; and Wahlby et al., An image analysis toolbox for high-throughput C. elegans assays, Nature Methods 9, 714-716 (2012).