Cytoplasm-to-nucleus translocation assay from the Broad Bioimage Benchmark Collection (BBBC013v1). 192 fluorescence microscopy images (640 x 640, 8-bit BMP) from one 96-well plate of human U2OS cells: 96 wells x 2 channels (channel 1 FKHR-GFP, channel 2 DNA stained with DRAQ), one field per well. The plate holds four replicate 9-point dose series of the PI3K inhibitors wortmannin (rows A-D, nM) and LY294002 (rows E-H, uM) plus 8 negative-control and 8 positive-control wells (150 nM wortmannin). Ground truth is the platemap: well, drug, dose and control role are stored as file metadata on every image and the control role is also an image-level label (negative-control, positive-control, dose). The two channels of each well are separate files paired through metadata and folder path; there are no cell outlines. License: CC BY 3.0 (Ilya Ravkin). Source: https://bbbc.broadinstitute.org/BBBC013. Please cite: We used image set BBBC013v1 provided by Ilya Ravkin, available from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012].